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Date of Publication:2015-01-01

Journal:植物生理学报

Volume:51

Issue:4

Page Number:509-516

ISSN No.:2095-1108

Abstract:Upf3 protein is one of the most important factors in the mRNA
   surveillance process. This process is a conservative mRNA degradation
   system which is mainly involved in the degradation of abnormal mRNA in
   eukaryotic cells. To study drought-tolerant genes, we compared the
   expression of Jingu34 under drought condition and normal one and
   filtrated drought-tolerant genes in the level of transcription. In this
   paper, we got the full length gene which is numbered Si009903m through
   searching the sequence within online Setaria italica genome data.
   Finally we cloned the coding sequence (CDS) of Si009903m and named it
   SiUPF3 for the Upf3 protein it encoded. The CDS of Si009903m is 1 515 bp
   and encodes 504 amino acids. Quantitative RT-PCR analysis infered that
   SiUPF3 is regulated by ABA signal network and significantly upregulated
   under drought.

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