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Indexed by:Journal Papers

Date of Publication:2019-10-01

Journal:ANALYTICAL CHEMISTRY

Included Journals:PubMed、EI、SCIE

Volume:91

Issue:19

Page Number:12531-12537

ISSN No.:0003-2700

Abstract:Cysteine (Cys) and homocysteine (Hcy) are essential for maintaining the cellular redox homeostasis and play critical roles in pathological and physiological processes. The development of Cys/Hcy-specific responsive fluorescent probes that are independent of the surrounding environment, equipment, and abundant endogenous GSH is critical to accurately investigate the roles of Cys/Hcy in living biological systems. In this work, a novel ratiometric and mitochondria-anchored fluorescence chemo-sensor, PYR, was constructed on the basis of 4-methylphenol-substituted pyronin fluorophore. The probe exhibited ratiometric fluorescence emission (F-540 (nm)/F-620 (nm)) for the detection of Cys/Hcy with high selectivity, sensitivity (Cys, 22 nM; Hcy, 23 nM), rapid response (Cys, 5 min), and a merit enhancement of ratio fluorescent signal (Cys, 163-fold; Hcy, 125-fold). The probe showed excellent membrane permeability and was applied to visualize mitochondrial biothiols in living cells under H2O2-induced redox imbalance, kidney tissues with a penetration depth of 100 mu m, and Daphnia magna model for the first time. The results demonstrate that PYR will provide a promising platform for the diagnosis of thiol-related diseases.