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论文类型：期刊论文

发表时间：2020-05-22

发表刊物：ACS SENSORS

收录刊物：SCIE

卷号：5

期号：5

页面范围：1411-1418

ISSN号：2379-3694

关键字：Singlet oxygen; two-photon fluorescence imaging; fluorescence lifetime imaging; photodynamic stress; phototoxicity

摘要：Singlet oxygen (O-1(2)) plays significant physiological and pathological functions, especially in causing photodynamic stress in vivo. However, specific O-1(2) monitoring is an immense challenge, owing to its short half-lives and high oxidizing ability. To address this, we engineered three photostable two-photon fluorescence probe NBs for highly efficient O-1(2) monitoring based on bioinspired novel tryptophan derivatives, among which NB-MOT was the best one comprehensively. Upon being cracked with O-1(2), NB-MOT rapidly (within 5 s) demonstrated a remarkable enhancement in fluorescence intensity (similar to 180 fold) and lifetime (similar to 18 fold). Taking these advantages into account, NB-MOT was applied to evaluate exogenous and endogenous O-1(2) in diverse biosystems. We successfully tracked the intracellular O-1(2) level during photodynamic therapy, and for the first time achieved O-1(2) mapping in live cells with dual-mode imaging as well as revealed ciprofloxacin-induced photodynamic stress in mice. NB-MOT was thus believed to be of instructive significance for studying the O-1(2)-mediated stress in wider biological milieus.