樊江莉

个人信息Personal Information

教授

博士生导师

硕士生导师

性别:女

毕业院校:大连理工大学

学位:博士

所在单位:化工学院

学科:应用化学. 精细化工

办公地点:大连理工大学西部校区F-206

联系方式:fanjl@dlut.edu.cn

电子邮箱:fanjl@dlut.edu.cn

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Dual mode monitoring probe for mitochondrial viscosity in single cell

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论文类型:期刊论文

发表时间:2014-01-01

发表刊物:SENSORS AND ACTUATORS B-CHEMICAL

收录刊物:SCIE、EI

卷号:190

页面范围:685-693

ISSN号:0925-4005

关键字:Near-infrared dye; Viscosity probe; Fluorescence ratio; Fluorescence lifetime; Mitochondrial viscosity; Apoptosis

摘要:Viscosity strongly influences intracellular transportation of mass and signal, and although bulk viscosity can be well measured with macroscopic samples quantities, microscale measurements in live cells still remain a challenge. By introducing a CHO group into the meso-position of the pentamethine chain, a thiazole-Cy5 dye based two-photon fluorescence probe, Mito-V, was developed. It exhibited a selective response to solution viscosity by fluorescence ratio and lifetime methods. More importantly, Mito-V can localize in mitochondria and quantitatively image its viscosity changes by the dual fluorescence mode during the apoptosis. (c) 2013 Elsevier B. V. All rights reserved.