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论文类型：期刊论文

发表时间：2012-07-20

发表刊物：ANALYTICA CHIMICA ACTA

收录刊物：SCIE、EI、PubMed

卷号：735

页面范围：107-113

ISSN号：0003-2670

关键字：BODIPY; Fluorescent probe; Cu2+; Oxidative dehydrogenation; Living cells

摘要：Based on a boron dipyrromethene (BODIPY) derivative containing an N, O and S tridentate ligand, a Cu2+ fluorescent probe BTCu was developed. The detection mechanism was verified as Cu2+-promoted oxidative dehydrogenation of an amine moiety, leading to a formation of a fluorescent Cu+-Schiff base complex. Free BTCu exhibited a maximum absorption wavelength at 496 nm, and a very weak maximum emission at 511 nm. Upon addition of various metals ions, it showed large fluorescence enhancement toward Cu2+ (417-fold in MeCN and 103-fold in MeCN/HEPES solution, respectively) with high selectivity. The detection limits are as low as 1.74 x 10(-8) M and 4.96 x 10(-8) M in the two different solutions, respectively. And BTCu could work in a wide pH range with an extraordinary low pK(a) of 1.21 +/- 0.06. Using fluorescence microscopy, the probe was shown to be capable of penetrating into living cells and imaging intracellular Cu2+ changes. (C) 2012 Elsevier B.V. All rights reserved.