点击次数：

论文类型：期刊论文

发表时间：2010-05-01

发表刊物：PROCESS BIOCHEMISTRY

收录刊物：SCIE、EI、Scopus

卷号：45

期号：5

页面范围：731-737

ISSN号：1359-5113

关键字：2,3-Butanediol; Aqueous two-phase extraction; Partition coefficient; Recovery; Fermentation

摘要：Separation of 2,3-butanediol from the fermentation broth is a difficult task that has become a bottleneck in industrial production. Aqueous two-phase systems composed of hydrophilic solvents and inorganic salts could be used to extract 2,3-butanediol from fermentation broth. The ethanol/ammonium sulfate system was investigated in detail, including phase diagram, effect of phase composition on partition, removal of cells and biomacromolecules from the broths and recycling of ammonium sulfate. The highest partition coefficient (7.10) and recovery of 2,3-butanediol (91.7%) were obtained by a system composed of 32% (w/w) ethanol and 16% (w/w) ammonium sulfate. The maximum selective coefficient of 2,3-butanediol to glucose was 30.74 in the experimental range. In addition, cells and proteins could be simultaneously removed from the fermentation broth. The removal ratio of cells and proteins reached 99.7% and 91.2%, respectively. The recovery of ammonium sulfate in the bottom phase reached 97.14% when two volumes of methanol were added to the salt-rich phase. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.