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Indexed by:期刊论文

Date of Publication:2016-12-01

Journal:ADVANCED SCIENCE

Included Journals:SCIE、EI、PubMed、Scopus

Volume:3

Issue:12

Page Number:1600146

ISSN No.:2198-3844

Key Words:europium complex,luminescence probe,protein detection,time‐resolved imaging

Abstract:A tetraphenylethene (TPE) group that exhibits aggregation-induced emission is incorporated into the ligand of a Eu(III) complex (TPEEu) to sensitize the excited state of Eu(III). In steady-state measurements, TPEEu exhibits weak luminescence when dissolved in aqueous solutions even at a high concentration level, but emits strong fluorescence of TPE and phosphorescence of Eu(III) upon binding with bovine serum albumin. With a delay time of 0.05 ms and a gate time of 1.0 ms in time-resolved measurements, only phosphorescent emission of Eu(III) is observed with a high on/off ratio. Moreover, this probe is successfully used in time-resolved luminescence imaging to eliminate the background signal from biological autofluorescence without a washing process. This work provides a general strategy in designing Ln(III) complexes for detecting a broad range of biological molecules.