Release Time:2019-03-12  Hits:

Indexed by: Journal Article

Date of Publication: 2016-10-04

Journal: ANALYTICAL CHEMISTRY

Included Journals: Scopus、PubMed、EI、SCIE

Volume: 88

Issue: 19

Page Number: 9564-9571

ISSN: 0003-2700

Abstract: We describe the application of a synthetically developed tetradentate beta-diketonate-europium chelate with high quantum yield (39%), for sensitive immunodetection of prostate cancer cells (DU145). MIL38 antibody, a mouse monoclonal antibody against Glypican 1, conjugated directly to the chelate via lysine residues, resulted in soluble (hydrophilic) and stable immunoconjugates. Indirect labeling of the antibody by a europium chelated secondary polyclonal antibody and a streptavidin/biotin pair was also performed. All of these bright luminescent conjugates were used to stain DU145 cells, a prostate cancer cell line, using time gated luminescence microscopy for imaging, and their performances were compared to conventional FITC labeling. For all prepared conjugates, the europium chelate in conjunction with a gated autosynchronous luminescence detector (GALD) completely suppressed the cellular autofluorescence background to allow capture of vivid, high contrast images of immune-stained cancer cells.