Hits:

Indexed by:期刊论文

Date of Publication:2011-06-01

Journal:ANALYTICAL CHEMISTRY

Included Journals:SCIE、EI、PubMed

Volume:83

Issue:11

Page Number:4163-4169

ISSN No.:0003-2700

Abstract:A highly sensitive Tb3(+) complex-based luminescent probe, N,N,N-1,N-1-[2,6-(3' - aminomethyl-1'-pyrazolyl)-4-(3 '',4 ''-diaminophenoxy)methylene-pyridine] tetralds(acetate)-Tb3+ (BMTA-Tb3+), has been designed and synthesized for the recognition and detection of hydrogen peroxide (H2O2) in aqueous solutions. This probe is almost nonluminescent because the Tb3+ luminescence is effectively quenched by the electron-rich moiety, diaminophenyl, on the basis of the photoinduced electron transfer (PET) mechanism. In the presence of peroiddase, the probe can react with H2O2 to cause the cleavage of the diaminophenyl ether, which affords a highly luminescent Tb3+ complex, N,N,N-1,N-1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-hydroxyinethyl-pyridine] tetralds-(acetate)-Tb3+ (BHTA-Tb3+), accompanied by a 39-fold increase in luminescence quantum yield with the increase of luminescence lifetime from 1.95 to 2.76 ms. The dose-dependent luminescence enhancement of the probe shows a good linearity with a detection limit of 3.7 nM for H2O2, which is approximately 14-fold lower than those of the commonly used fluorescent probes. The probe was used for the time-resolved luminescence imaging detection of the oligosaccharide-induced H2O2 generation in tobacco leaf epidermal tissues. On the basis of the probe, a background-free time-resolved luminescence imaging method for detecting H2O2 in complicated biological systems was successfully established.