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Indexed by:期刊论文

Date of Publication:2011-05-15

Journal:TALANTA

Included Journals:SCIE、EI、PubMed

Volume:84

Issue:3

Page Number:971-976

ISSN No.:0039-9140

Key Words:Terbium chelate; Luminescent probe; Hydroxyl radical; Time-resolved luminescence; Cell imaging

Abstract:Time-resolved (or time-gated) luminescence detection technique using lanthanide chelates as luminescent probes is a widely used and highly sensitive method for the biological applications. The developments of various functional lanthanide probes that can selectively recognize the biological targets are the essential objective of the technique. In this work, a unique Tb(3+) chelate-based luminescent probe, N,N,N(1),N(1)-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-(p-aminophenoxy)methylene-pyridine] tetrakis(acetate)-Tb(3+)(BMPTA-Tb(3+)), has been designed and synthesized for highly selective and sensitive time-resolved luminescence detection of one highly reactive oxygen species (ROS), hydroxyl radical ((center dot)OH). The probe is almost non-luminescent, and can selectively react with hydroxyl radical to afford a highly luminescent Tb(3+) chelate, N,N,N(1),N(1)-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-hydroxymethyl-pyridine] tetrakis(acetate)-Tb(3+) (BHTA-Tb(3+)), accompanied by a 49-fold increase in luminescence quantum yield with a long luminescence lifetime (2.76ms). The luminescence response of the probe to hydroxyl radical is highly selective and insensitive to pH in the physiological pH range. For loading the probe into the living cells, the acetoxymethyl ester of BMPTA-Tb(3+) was synthesized and used for the HeLa cell loading. Based on this probe, a background-free time-resolved luminescence imaging method for detecting hydroxyl radical in living cells was successfully established. (C) 2011 Elsevier B.V. All rights reserved.