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论文类型：期刊论文

发表时间：2016-03-01

发表刊物：INORGANIC CHEMISTRY COMMUNICATIONS

收录刊物：SCIE

卷号：65

页面范围：28-31

ISSN号：1387-7003

关键字：Fluorescence probe; Copper; Naphthalimide; Cell imaging

摘要：Anew fluorescence probe L based on naphthalimide has been synthesized for selective and quantitative detection of Cu2+ in CH3CN:H2O (4:1, v/v) solution. L exhibited a strong green fluorescence. Upon addition of 2 equiv. of Cu2+, the fluorescence emission shows a steady and smooth decrease until a plateau is reached with a 30-fold quenching of fluorescence intensity. In the presence of Cu2+, the absorbance peak of L maximum at 466 nm decreased, and a new absorption band at 600 nm appeared. Under the identical conditions, other physiological and environmental important metal ions induced negligible spectroscopic changes. The 1:2 stoichiometry binding mode of L with Cu2+ was supported by the Benesi-Hildebrand analysis and ESI-MS spectra studies. The detection limit for Cu2+ was estimated to be 64 ppb. Fluorescence microscopy experiments showed that L has practical application in living cells. (C) 2015 Elsevier B.V. All rights reserved.