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Indexed by:期刊论文

Date of Publication:2016-02-01

Journal:MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS

Included Journals:EI、SCIE、Scopus

Volume:22

Issue:2

Page Number:283-286

ISSN No.:0946-7076

Abstract:In this paper, a method of electrokinetic concentrating and a glass nanofluidic device for sensitivity-enhancing of magnetic beads-based antigen-antibody immunoassay (MBAAI) are presented. In the nanofluidic device, a polyacrylamide gel plug is deployed as the nanopores via photopolymerization reaction. To verify the capability of electrokinetic concentrating, the fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) with initial concentration of 0.002 ng/ml is enriched and the concentration factor of a hundred million fold is achieved. And then the technique is implemented to improve the MBAAI sensitivity. In this experiment, magnetic beads coupled with sheep anti-rabbit IgG (immunoglobulin G) are firstly trapped into the immunoassay region via magnetic force, then the FITC-labeled rabbit IgG is electrokinetically concentrated and spontaneously bonded with the previous fixed magnetic beads. After the incubation, the total fluorescence intensity in immunoassay region is calculated. The result shows that the fluorescence intensity is improved by 58 % and the antigen-concentrating with the nanofluidic device can effectively enhance the sensitivity of MBAAI.