location: Current position: Home >> Scientific Research >> Paper Publications

The use of a near-infrared RNA fluorescent probe with a large Stokes shift for imaging living cells assisted by the macrocyclic molecule CB7

Hits:

Indexed by:期刊论文

Date of Publication:2013-09-01

Journal:BIOMATERIALS

Included Journals:SCIE、EI、PubMed、Scopus

Volume:34

Issue:27

Page Number:6473-6481

ISSN No.:0142-9612

Key Words:NIR dye; RNA probe; Fluorescent imaging; Large Stokes shift; Cucurbit[7]uril

Abstract:A near-infrared fluorescent dye Hsd was designed and synthesized, which absorbed as hemicyane and emitted as Cy7 and therefore produced a Stokes shift as large as 224 nm. Quantum chemistry calculation demonstrated that the large Stokes shift was produced by the combination of intramolecular charge transfer (ICT) and internal conversion. Significantly, Hsd showed selectively response to RNA in aqueous solution and fixed cells. Moreover, Hsd could be uptaken into the cells under the assistance of cucurbit[7]uril (CB7) and selectively stain RNA in living cells. The introducing of CB7 provides a platform to amplify the application of some cell-impermeant fluorescent stains through the supramolecular chemistry methods. (C) 2013 Elsevier Ltd. All rights reserved.

Pre One:Interaction of Ru(phen)(3)Cl-2 with graphene oxide and its application for DNA detection both in vitro and in vivo

Next One:连接链长对双核钌分子内与分子间协同 ECL影响研究