Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2013-09-07

Journal: JOURNAL OF MATERIALS CHEMISTRY B

Included Journals: Scopus、SCIE、EI

Volume: 1

Issue: 33

Page Number: 4146-4151

ISSN: 2050-750X

Abstract: The fluorescence of Ru(phen)(3)Cl-2 (tris(1,10-phenanthroline) ruthenium(II) dichloride) can be effectively quenched with the addition of graphene oxide in aqueous solution, due to the formation of a GO-Ru hybrid. A fluorescence enhancement of approximately 50 times can be observed after the addition of a certain amount of DNA into the above-mentioned solution. The fluorescence increase is linearly proportional to the amount of DNA added in the concentration range of 0-70 mu M and the DNA detection limit is down to 3.3 x 10(-8) M. Meanwhile, it is found that the GO-Ru hybrid can enter into the nuclei and stain the DNA of living human breast cancer cells MCF-7, while Ru(phen)(3)Cl-2 alone cannot cross the cellular membrane in the control experiment. This method can be employed to detect DNA both in vitro and in vivo.