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Date of Publication:2022-10-10

Journal:催化学报

Affiliation of Author(s):生物工程学院

Volume:35

Issue:9

Page Number:1534-1546

ISSN No.:0253-9837

Abstract:Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gar-denia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extrac-tion of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8%after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0-5.0. The Km and Vmax of the enzyme for geniposide were 3.6 mmol/L and 775μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin.

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