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Date of Publication:2022-10-06

Journal:化工学报

Issue:6

Page Number:1877-1882

ISSN No.:0438-1157

Abstract:Using coupling biotech of cellulase-catalysis and extrusion in three-liquid-phase system that composed of n-hexane, 1, 4-dioxane and ammonium sulfate to hydrolyze dioscin in Chinese yan and glucose connected to steroidal saponins, and extration separation to collect diosgenin. The distribution of several steroidal saponins, hydrolyzed glucose, enzyme and its activity in the three-phase system were studied, and catalytic conversion by cellulase-enzyme and formation of diosgenin in three-liquid, organic and aqueous phases were compared. The results showed that diosgenin yield in the three-liquid-phase system could compare with that in organic phase and aqueous phase. The three-liquid-phase system could be suitable for simultaneous enzymatic hydrolysis of steroidal saponins and extration of diosgenin and glucose. The substrate and enzyme were held in the middle phase of 1, 4-dioxane, whiles the products, diosgenin and glucose, were partitioned into the top and bottom phase, respectively. The yields of diosgenin was 69.4% at 96 h in the three-liquid-phase system, which was twice and 27.6 folds obtained in organic phase and aqueous phase respectively.

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