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Date of Publication:2022-10-06

Journal:中草药

Issue:2

Page Number:223-227

ISSN No.:0253-2670

Abstract:Objective To isolate and purify resveratrol fromermentation broth of Polygonum cuspi-datum. Methods Macroporous resin and polyamide column chromatography were combined to purify res- veratrol from the extract in fermentation broth of P. cuspidatum, and the adsorption dynamics of resvera-trol was evaluated. Results Apparent static adsorptive capacities of resveratrol on macroporous resin and polyamide were 11.70 and 57.9 mg/g, respectively, and apparent dynamic adsorptive capacities were 12.47 and 58.98 mg/g,respectively. Both of the adsorption isotherms correlate well with Langmuir type. After macroporous resin and polyamide two-step column chromatography, the purity of resveratro] reached 95.8%, with the yield of 69.8%. Conclusion It is proved the method is potential for industrial applica-tion clue to its simple procedure, lower cost as well as high purity and yield of resveratrol.

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