吴硕

个人信息Personal Information

教授

博士生导师

硕士生导师

主要任职:化学学院副院长

其他任职:辽宁省教学名师,宝钢优秀教师特等奖提名奖,中国分析测试学会青年学术委员会委员

性别:女

毕业院校:南京大学

学位:博士

所在单位:化学学院

学科:分析化学. 生物化学与分子生物学

办公地点:化工综合楼C305

电子邮箱:wushuo@dlut.edu.cn

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Ultrasensitive enzyme-free electrochemical immunosensor based on hybridization chain reaction triggered double strand DNA@Au nanoparticle tag

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论文类型:期刊论文

发表时间:2014-03-01

发表刊物:TALANTA

收录刊物:SCIE、EI、PubMed

卷号:120

页面范围:218-223

ISSN号:0039-9140

关键字:Electrochemical immunosensor; Signal amplification; Hybridization chain reaction; Carcinoembryonic antigen; Hexaammineruthenium

摘要:An ultrasensitive enzyme-free electrochemical immunoassay was developed for detection of the fg/mL level carcinoembryonic antigen (CEA) by using a double strand DNA@Au nanoparticle (dsDNA@AuNP) tag and hexaammineruthenium(III) chloride (RuHex) as the electroactive indicator. The dsDNA@AuNP was synthesized by one-pot hybrid polymerization of dsDNA on initiator DNA modified AuNPs via hybridization chain reaction. The immunosensor was prepared by covalently cross-linking capture antibody on chitosan/AuNP nanocomposite modified glass carbon electrode. The AuNPs accelerated the electron transfer and led to high detection sensitivity. With a sandwich-type immunoreaction and a biotin-streptavidin affinity reaction, the dsDNA@AuNP tag was conjugated on the immunocomplex to bring a high amount of RuHex to the electrode surface via electrostatic interaction, resulting in an amplified electrochemical signal. Under optimal conditions, the proposed sensing platform showed a wide linear detection range from 10 fg/mL to 10 ng/mL along with a detection limit of 3.2 fg/mL for CEA. The immunosensor exhibited high sensitivity and good stability, showing a promising application in early cancer diagnosis and could be extended to sensitive electrochemical biosensing of other analytes. (C) 2013 Elsevier B.V. All rights reserved.