个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职:化学学院副院长
其他任职:辽宁省教学名师,宝钢优秀教师特等奖提名奖,中国分析测试学会青年学术委员会委员
性别:女
毕业院校:南京大学
学位:博士
所在单位:化学学院
学科:分析化学. 生物化学与分子生物学
办公地点:化工综合楼C305
电子邮箱:wushuo@dlut.edu.cn
Amplified Single Base-Pair Mismatch Detection via Aggregation of Exonuclease-Sheared Gold Nanoparticles
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论文类型:期刊论文
发表时间:2014-04-01
发表刊物:ANALYTICAL CHEMISTRY
收录刊物:SCIE、EI、Scopus
卷号:86
期号:7
页面范围:3461-3467
ISSN号:0003-2700
摘要:Single nucleotide polymorphism (SNP) detection is important for early diagnosis, clinical prognostics, and disease prevention, and a rapid and sensitive low-cost SNP detection assay would be valuable for resource-limited clinical settings. We present a simple platform that enables sensitive, naked-eye detection of SNPs with minimal reagent and equipment requirements at room temperature within 15 min. SNP detection is performed in a single tube with one set of DNA probe-modified gold nanoparticles (AuNPs), a single exonuclease (Exo III), and the target in question. Exo III's apurinic endonucleolytic activity differentially processes hybrid duplexes between the AuNP-bound probe and DNA targets that are perfectly matched or contain a single-base mismatch. For perfectly matched targets, Exo III's exonuclease activity facilitates a process of target recycling that rapidly shears DNA probes from the particles, generating an AuNP aggregation-induced color change, whereas no such change occurs for mismatched targets. This color change is easily observed with as little as 2 nM of target, 100-fold lower than the target concentration required for reliable naked eye observation with unmodified AuNPs in well-optimized reaction conditions. We further demonstrate that this system can effectively discriminate a range of different mismatches.