吴硕

个人信息Personal Information

教授

博士生导师

硕士生导师

主要任职:化学学院副院长

其他任职:辽宁省教学名师,宝钢优秀教师特等奖提名奖,中国分析测试学会青年学术委员会委员

性别:女

毕业院校:南京大学

学位:博士

所在单位:化学学院

学科:分析化学. 生物化学与分子生物学

办公地点:化工综合楼C305

电子邮箱:wushuo@dlut.edu.cn

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Controlled immobilization of acetylcholinesterase on improved hydrophobic gold nanoparticle/Prussian blue modified surface for ultra-trace organophosphate pesticide detection

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论文类型:期刊论文

发表时间:2011-09-15

发表刊物:BIOSENSORS & BIOELECTRONICS

收录刊物:Scopus、SCIE、EI、PubMed

卷号:27

期号:1

页面范围:82-87

ISSN号:0956-5663

关键字:Acetylcholinesterase; Orientated immobilization; Improved hydrophobic interface; Organophosphate pesticide; Ultra-low limit of detection

摘要:An ultrasensitive amperometric acetylcholinesterase (AChE) biosensor was fabricated by controlled immobilization of AChE on gold nanoparticles/poly(dimethyldiallylammonium chloride) protected Prussian blue (Au-PDDA-PB) nanocomposite modified electrode surface for the detection of organophorous pesticide. The Au-PDDA-PB membrane served as an excellent matrix for the immobilization of enzyme, which not only enhanced electron transfer but also possessed a relatively large surface area. In addition, the surface hydrophilicity of the Au-PDDA-PB nanocomposite was finely controlled in the static water contact angle range of 25.6-78.1 degrees by adjusting the ratio of gold nanoparticles to PDDA-PB. On an optimized hydrophobic surface, the AChE adopts an orientation with both good activity and stability, which has been proven by electrochemical methods. Benefit from the advantages of the Au-PDDA-PB nanocomposite and the good activity and stability of AChE, the biosensor shows significantly improved sensitivity to monocrotophos, a typical highly toxic organophorous pesticide, with wide linear range (1.0-1000 pg/mL and 1.0-10 ng/mL) and an ultra-low detection limit of 0.8 pg/mL. The biosensor exhibits accuracy, good reproducibility and stability. This strategy may therefore provide useful information for the controlled immobilization of protein and the design of highly sensitive biosensors. (C) 2011 Elsevier B.V. All rights reserved.