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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：化学学院副院长
其他任职：辽宁省教学名师，宝钢优秀教师特等奖提名奖，中国分析测试学会青年学术委员会委员
性别：女
毕业院校：南京大学
学位：博士
所在单位：化学学院
学科：分析化学. 生物化学与分子生物学
办公地点：化工综合楼C305
电子邮箱：wushuo@dlut.edu.cn
Magnetic loading of tyrosinase-Fe3O4/mesoporous silica core/shell microspheres for high sensitive electrochemical biosensing
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论文类型：期刊论文
发表时间：2011-02-07
发表刊物：ANALYTICA CHIMICA ACTA
收录刊物：Scopus、SCIE、EI、PubMed
卷号：686
期号：1-2
页面范围：81-86
ISSN号：0003-2670
关键字：Tyrosinase; Fe3O4/mSiO(2) core/shell mesoporous; microspheres; Magnetic loading; Flow injection analysis; Amperometry
摘要：A new protocol is proposed for magnetic loading and sensitive electrochemical detection of phenol via the tyrosinase cross-linked mesoporous magnetic core/shell microspheres. The mesoporous magnetic microspheres, characterized by transmission electron microscopy, N-2 adsorption/desorption isotherms, and magnetic curve displays high capacity for enzyme immobilization and strong magnetism to adhere to the magnetic electrode surface without any additional adhesive reagent. The biosensor exhibits a wide linear response to phenol ranging from 1.0 x 10(-9) to 1.0 x 10(-5) M, a high sensitivity of 78 mu A mM(-1), a low detection limit of 1 nM, and a fast response rate (less than 5 s). The proposed method is simple, rapid, inexpensive and convenient in electrode renewal, which is recommended as a promising experimental platform for wider applications in biosensing. (C) 2010 Elsevier B.V. All rights reserved.