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论文类型：期刊论文

发表时间：2018-01-01

发表刊物：Food chemistry

收录刊物：PubMed、SCIE、EI

卷号：258

页面范围：87-94

ISSN号：0308-8146

关键字：Antibiotic; Sea cucumber; ASE; UPLC-MS/MS; In-cell clean-up

摘要：An accelerated solvent extraction (ASE) with in-cell clean-up method coupled to ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to determine 21 antibiotics in sea cucumber. The analytes include 10 sulfonamides, 4 fluoroquinolones, 3 amphenicols, 2 beta-lactams, 1 lincosamide and trimethoprim. Optimal parameters of ASE method were obtained at 80 °C, 1 static cycle of 5 min with methanol/acetonitrile (1/1, v/v) using 2 g of C18 as adsorbent. Recoveries at 50.1-129.2% were achieved with RSD under 20%. Method detection limits ranged from 0.03 to 2.9 mug kg-1. Compared to the reported ultrasound-assisted extraction method, the proposed method offered comparable extraction efficiency for sulfonamides from sea cucumber, but higher for other categories of antibiotics. This validated method was then successfully applied to sea cucumber samples and 9 antibiotics were detected with the highest concentration up to 57.7 mug kg-1 for norfloxacin. Copyright © 2018 Elsevier Ltd. All rights reserved.