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论文类型：期刊论文

发表时间：2017-02-01

发表刊物：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT

收录刊物：SCIE、EI、PubMed、Scopus

卷号：34

期号：2

页面范围：255-264

ISSN号：1944-0049

关键字：Mycotoxins; vegetable oil; QuEChERS; HPLC-MS; MS

摘要：A simple and efficient method for determining multiple mycotoxins was developed using a QuEChERS (quick, easy, cheap, effective, rugged and safe)-based extraction procedure in vegetable oils. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used for the quantification and confirmation of 16 chemically diversified mycotoxins. Different extraction procedures were studied and optimised by spiking 16 analytes into blank matrix, and the extraction with 85% MeCN solution and C18 as cleaning sorbent allowed an efficient recovery of 72.8-105.8% with RSDs less than 7%. The limit of detection (LOD) ranged from 0.04 to 2.9 ngg(-1). The developed method was finally applied to screen mycotoxins in 62 vegetable oil samples. Zearalenone (ZEN), aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and -zearalenol (-ZOL) were detected, with maximum concentrations of 0.59 (AFG1)-42.5 (ZEN) ngg(-1). The method developed has the advantages of high sensitivity, accuracy and selectivity, and it can be applied to the target screening of mycotoxins in real samples.