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Indexed by:期刊论文

Date of Publication:2019-01-28

Journal:CHEMISTRY-A EUROPEAN JOURNAL

Included Journals:SCIE、PubMed、Scopus

Volume:25

Issue:6

Page Number:1498-1506

ISSN No.:0947-6539

Key Words:cysteine; iridium; phosphorescence; sensing and imaging; time-gated luminescence

Abstract:This study reports an activatable iridium(III) complex probe for phosphorescence/time-gated luminescence detection of cysteine (Cys) in vitro and in vivo. The probe, [Ir(ppy)(2)(NTY-bpy)](PF6) [ppy: 2-phenylpyridine; NTY-bpy: 4-methyl-4 '-(2-nitrovinyl)-2,2 '-bipyridine], is developed by incorporating a strong electron-withdrawing group, nitroolefin, into a bipyridine ligand of the Ir-III complex. The luminescence of the probe is quenched owing to the intramolecular charge transfer (ICT) process, but switched on by a specific recognition reaction between the probe and Cys. [Ir(ppy)(2)(NTY-bpy)](PF6) shows high sensitivity and selectivity for Cys detection and good biocompatibility. The long-lived emission of [Ir(ppy)(2)(NTY-bpy)](PF6) allows time-gated luminescence analysis of Cys in cells and human sera. These properties make it convenient for the phosphorescence and time-gated luminescence imaging and flow cytometry analysis of Cys in live samples. The Cys images in cancer cells and inflamed macrophage cells reveal that [Ir(ppy)(2)(NTY-bpy)](PF6) is distributed in mitochondria after cellular internalization. Visualizations and flow cytometry analysis of mitochondrial Cys levels and Cys-mediated redox activities of live cells are achieved. By using [Ir(ppy)(2)(NTY-bpy)](PF6) as a probe, in vivo sensing and imaging of Cys in D. magna, zebrafish, and mice are then demonstrated.