location: Current position: Home >> Scientific Research >> Paper Publications

测定饮料中咖啡因含量的综合性实验

Hits:

Indexed by:期刊论文

Date of Publication:2016-01-15

Journal:实验室研究与探索

Included Journals:PKU

Volume:35

Issue:1

Page Number:13-15

ISSN No.:1006-7167

Key Words:咖啡因;高效液相色谱法;提取

Abstract:结合有机化学实验内容,采用蒸馏法用乙醇溶剂从茶叶中提取咖啡因晶体,用高效液相色谱仪考察了流动相组成对样品分离效果的影响,发现增大甲醇浓度,咖啡因的保留时间随之减少.咖啡因浓度20~100 μg/mL,线性方程为A=2.38C +33.4,R2=0.991.最后,通过高效液相色谱法(HPLC)测定市售绿茶饮料、可乐和功能饮料中的咖啡因含量,所得色谱图中无干扰叠加峰,并且在几种饮料中均含有一定量的咖啡因,建议需适量摄取含咖啡因的饮料.

Pre One:A unique iridium(III) complex-based chemosensor for multi-signal detection and multi-channel imaging of hypochlorous acid in liver injury

Next One:转基因产品分析方法的研究进展