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Indexed by:Journal Papers

Date of Publication:2015-05-01

Journal:CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY

Included Journals:SCIE、ISTIC、Scopus

Volume:33

Issue:3

Page Number:705-713

ISSN No.:0254-4059

Key Words:glutathione S-transferase (GST); Venerupis philippinarum; Mytilus galloprovincialis; 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47); molecular docking; biomarker

Abstract:Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs (VpGSTs) and four Mytilus galloprovincialis GSTs (MgGSTs) following exposure to BDE-47. Differential expressions of the seven VpGSTs and four Mg GSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes (VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3) were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking. Hydrophobic and pi were the most often observed interactions between BDE-47 and the GSTs.