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Indexed by:期刊论文

Date of Publication:2017-05-01

Journal:SENSORS AND ACTUATORS B-CHEMICAL

Included Journals:SCIE、EI、Scopus

Volume:243

Page Number:1255-1260

ISSN No.:0925-4005

Key Words:Metal-waveguide-capillary based photometer; Aptamer; Gold nanorods; Thrombin

Abstract:This work described a sensitive thrombin analysis method employing metal-waveguide-capillary (MWC) photometry with a sandwich aptamer-gold nanorods (GNRs) as a signal transporting probe. The sandwich aptasensor consisted of a pair of thrombin-binding aptamers: one 29-mer aptamer modified with magnetic beads as capture aptamers; and one 15-mer aptamer binding with GNRs as report aptamers. Through the measurement of GNRs from the sandwich complex, the MWC photometry enabled to detect thrombin with a linear range from 0.068 nM to 1.09 nM, and with a detection limit as low as 0.02 nM. The detection limit was improved 68-fold vs. that of commercial spectrophotometer with 1 cm cuvette. (C) 2016 Elsevier B.V. All rights reserved.