Enabling the Triplet of Tetraphenylethene to Sensitize the Excited State of Europium(III) for Protein Detection and Time-Resolved Luminescence Imaging
Release Time:2019-03-12 Hits:
Indexed by: Journal Article
Date of Publication: 2016-12-01
Journal: ADVANCED SCIENCE
Included Journals: Scopus、PubMed、EI、SCIE
Volume: 3
Issue: 12
Page Number: 1600146
ISSN: 2198-3844
Key Words: europium complex,luminescence probe,protein detection,time‐resolved imaging
Abstract: A tetraphenylethene (TPE) group that exhibits aggregation-induced emission is incorporated into the ligand of a Eu(III) complex (TPEEu) to sensitize the excited state of Eu(III). In steady-state measurements, TPEEu exhibits weak luminescence when dissolved in aqueous solutions even at a high concentration level, but emits strong fluorescence of TPE and phosphorescence of Eu(III) upon binding with bovine serum albumin. With a delay time of 0.05 ms and a gate time of 1.0 ms in time-resolved measurements, only phosphorescent emission of Eu(III) is observed with a high on/off ratio. Moreover, this probe is successfully used in time-resolved luminescence imaging to eliminate the background signal from biological autofluorescence without a washing process. This work provides a general strategy in designing Ln(III) complexes for detecting a broad range of biological molecules.