Hits:

Indexed by:期刊论文

Date of Publication:2013-11-01

Journal:JOURNAL OF FLUORESCENCE

Included Journals:SCIE、EI、PubMed、Scopus

Volume:23

Issue:6

Page Number:1113-1120

ISSN No.:1053-0509

Key Words:Ruthenium complex; Luminescent probe; Nitric oxide; Luminescence detection

Abstract:Nitric oxide (NO) is one of the most important intercellular signaling molecules, and plays important roles in various biological systems. In this work, a unique Ru-II complex, tris[(5-(4-methylamino-3-aminobenzylamino)-1,10-phenanthroline)] ruthenium(II) hexafluorophosphate [Ru(MAA-phen)(3)][PF6](2), has been designed and synthesized as a luminescent probe for the detection of NO in aqueous media. The complex itself is almost non-luminescent, but can specifically react with NO under the aerobic conditions to afford its highly luminescent triazole derivative in aqueous media, [Ru(MTA-phen)(3)](2+) (MTA-phen: methyl-trazolebenzylamino-1,10-phenanthroline), accompanied by a 302-fold increase in luminescence intensity at 598 nm with a 130 nm Stokes shift. The luminescence response of [Ru(MAA-phen)(3)](2+) to NO is rapid, highly specific without interferences of other reactive oxygen/nitrogen species, and highly stable against the pH changes in the range of pH 4.5-9.5. These features enable [Ru(MAA-phen)(3)](2+) to be used as a probe for the highly selective and sensitive luminescence detection of NO in weakly acidic, neutral, and weakly basic media.