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Indexed by:期刊论文

Date of Publication:2013-04-15

Journal:TALANTA

Included Journals:SCIE、EI、PubMed

Volume:108

Page Number:143-149

ISSN No.:0039-9140

Key Words:Visible-light-excited europium biolabel; Transferrin; Folic acid; Time-resolved luminescence bioassay; Cell imaging

Abstract:By using a visible-light-excited ternary Eu3+ complex, BHHBCB-Eu3+-BPT (BHHBCB: 1,2-bis[4'-(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl)-benzyl]-4-chlorosulfobenzene; BPT: 2-(N,N-diethylanilin-4-yl)-4,6-bis(pyrazol-1-yl)-1,3,5-triazine), as a luminophore, two kinds of novel visible-light-excited europium materials, the silica-encapsulated BHHBCB-Eu3+-BPT (Eu@SiO2) nanoparticles and BHHBCB-Eu3+-BPT-conjugated bovine serum albumin (BSA-BHHBCB-Eu3+-BPT), were prepared for biolabeling and time-resolved luminescence cell imaging applications. The Eu@SiO2 nanoparticles, prepared by the copolymerization of 3-aminopropyl(triethoxy)silane-BHHBCB-Eu3+-BPT conjugate, free 3-aminopropyl(triethoxy) silane and tetraethyl orthosilicate in a water-in-oil reverse microemulsion, are monodispersed, spherical and uniform in size, and strongly luminescent with an excitation peak at similar to 400 nm and a long luminescence lifetime of 346 mu s. The BSA-BHHBCB-Eu3+-BPT, prepared by covalent binding of BHHBCB-Eu3+-BPT to BSA, shows also strong visible-light-excited luminescence with a excitation peak at similar to 400 nm and a long luminescence lifetime of 402 mu s. The two materials were used for labeling transferrin and folic acid. Using the time-resolved luminescence imaging of living HeLa cells, the cell-surface receptors of transferrin and folic acid were successfully visualized by the prepared biolabels based on the ligand-receptor affinity binding interaction. The results demonstrated the feasibility of the new materials as visible-light-excited biolabels for the time-resolved luminescence cell imaging. (C) 2013 Elsevier B.V. All rights reserved.