宋波

个人信息Personal Information

副教授

博士生导师

硕士生导师

性别:男

毕业院校:大连化物所

学位:博士

所在单位:化学学院

学科:分析化学

办公地点:大连理工大学西部校区化工综合楼D313

联系方式:+86-411-84986042

电子邮箱:bo.song@dlut.edu.cn

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Background-free in-vivo Imaging of Vitamin C using Time-gateable Responsive Probe

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论文类型:期刊论文

发表时间:2015-09-16

发表刊物:SCIENTIFIC REPORTS

收录刊物:SCIE、PubMed、Scopus

卷号:5

页面范围:14194

ISSN号:2045-2322

摘要:Sensitive optical imaging of active biomolecules in the living organism requires both a molecular probe specifically responsive to the target and a high-contrast approach to remove the background interference from autofluorescence and light scatterings. Here, a responsive probe for ascorbic acid (vitamin C) has been developed by conjugating two nitroxide radicals with a long-lived luminescent europium complex. The nitroxide radical withholds the probe on its "off" state (barely luminescent), until the presence of vitamin C will switch on the probe by forming its hydroxylamine derivative. The probe showed a linear response to vitamin C concentration with a detection limit of 9.1 nM, two orders of magnitude lower than that achieved using electrochemical methods. Time-gated luminescence microscopy (TGLM) method has further enabled real-time, specific and background-free monitoring of cellular uptake or endogenous production of vitamin C, and mapping of vitamin C in living Daphnia magna. This work suggests a rational design of lanthanide complexes for backgroundf-ree small animal imaging of biologically functional molecules.