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论文类型：期刊论文

发表时间：2013-12-15

发表刊物：BIOSENSORS & BIOELECTRONICS

收录刊物：SCIE、PubMed、Scopus

卷号：50

页面范围：1-7

ISSN号：0956-5663

关键字：Phosphorescent chemosensor; Ruthenium(II) complex; Hypochlorous acid; Luminescence imaging

摘要：Microbicidal activity of hypochlorous acid (HOCl) plays pivotal roles in many biological processes, and suitable methods for the in vivo detection of HOCl are highly desirable. Herein we report the development of two novel ruthenium(II) complex-based phosphorescent chemosensors for HOG, [Ru(bPY)(2)(DNCA-bPY)](PF6)(2) (bpy: 2,2'-bipyridine; DNCA-bpy: 4-N-(2,4-dinitrophenyl)carboxamide-4'methyl-2,2'-bipyridine) and [Ru(bpy)(2)(DNCH-bpy)](PF6)(2) (DNCH-bpy: 4-N-(2,4-dinitrophenyecarbohydrazide-4'-methyl-2,2'-bipyridine). The two sensors are almost non-luminescent due to the intramolecular photoinduced electron transfer (PET) process from the Ru(II)-bpy moiety to dinitrophenyl group, but specific reaction of the sensors with HOCl in aqueous media can afford a highly luminescent derivative, [Ru(bpy)(2)(COOH-bpy)](PF6)(2) (COOH-bpy: 4-carboxylic acid-4'-methyl-2,2'-bipyridine), accompanied by 190-fold and 1100-fold turn-on luminescence signal enhancement for [Ru(bpy)z(DNCA-bpY)](PF6)(2) and [Ru(bPY)(2)(DNCH-bPY)](PF6)(2), respectively. By taking advantage of high specificity and sensitivity of the chemosensor and excellent photophysical property of Ru(II) complex, [Ru(bpy)(2)(DNCH-bpY)](PF6)(2) was applied for visualizing the endogenous HOCl generation in living macrophage cells during the processes of stimulation and phagocytosis via the red-emitting luminescence. (C) 2013 Elsevier B.V. All rights reserved.