宋波

个人信息Personal Information

副教授

博士生导师

硕士生导师

性别:男

毕业院校:大连化物所

学位:博士

所在单位:化学学院

学科:分析化学

办公地点:大连理工大学西部校区化工综合楼D313

联系方式:+86-411-84986042

电子邮箱:bo.song@dlut.edu.cn

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A Lanthanide Complex-Based Ratiometric Luminescence Probe for Time-Gated Luminescence Detection of Intracellular Thiols

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论文类型:期刊论文

发表时间:2013-12-03

发表刊物:ANALYTICAL CHEMISTRY

收录刊物:SCIE、EI、PubMed、Scopus

卷号:85

期号:23

页面范围:11658-11664

ISSN号:0003-2700

摘要:A lanthanide complex-based ratiometric luminescence probe, [4'-(2,4-dinitrobenzenesulfonyloxy)-2,2':6',2 ''-terpyridine-6,6 ''-diyl] bis(methylenenitrilo) tetrakis(acetate)-Eu3+/Tb3+ (NSTTA-Eu3+/Tb3+), has been designed and synthesized for the specific recognition and time-gated luminescence detection of biothiols in physiological pH aqueous media. The probe itself is almost nonluminescent due to the presence of photoinduced electron transfer (PET) from the terpyridine-Ln(3+) moiety to the 2,4-dinitrobenzene-sulfonyl (DNBS) moiety. In the presence of biothiols, the reaction of NSTTA-Eu3+/Tb3+ with biothiols results in the cleavage of DNBS to afford the deprotonated (4'-hydroxy-2,2':6',2 ''-terpyridine-6,6 ''-diyl) bis(methylenenitrilo) tetrakis(acetate)-Eu3+/Tb3+ (HTTA-Eu"/Tb3+), which terminates the PET process. After the reaction, the intensity of Eu3+ emission at 610 nm is unchanged, while that of Tb3+ emission at 540 nm is remarkably increased, which provides a similar to 36-fold enhanced intensity ratio of Tb3+ emission to Eu3+ emission (I-540/I-610. This unique luminescence response allows NSTTA-Eu3+/Tb3+ to be used as a ratiometric probe for the time-gated luminescence detection of biothiols, using the intensity ratio of I-540/I-610 as a signal. Thus, based on the probe NSTTA-Eu3+/Tb3+, a ratiometric time-gated luminescence detection method for biothiols was established and successfully used for the quantitative detection of the total biothiols in several living cell samples.