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论文类型： 期刊论文

发表时间： 2017-10-01

发表刊物： CHINESE CHEMICAL LETTERS

收录刊物： SCIE、Scopus

卷号： 28

期号： 10,SI

页面范围： 1991-1993

ISSN号： 1001-8417

关键字： Biomarker; Fluorescent probe; alpha-Ketoglutaricacid; Actue myeloid leukemia (AML); Serum detection

摘要： alpha-Ketoglutaric acid (alpha-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body. The mutations of isocitrate dehydrogenase-1 (IDH1) and isocitrate dehydrogenase-2 (IDH2), however, will lead to the transformation of alpha-KA into 2-hydroxyglutarate (2-HG), which is confirmed to closely related to actue myeloid leukemia (AML). Therefore it is of great significance to detect alpha-KA level changes in serum. In this paper, a fluorescent "off-on" probe CH for alpha-KA was designed based on naphthalimide fluorophore by introducing a hydrazine group for alpha-KA recognition and a long alkyl amino chain to enhance PET efficiency and water solubility. Cetyltrimethyl ammonium bromide (CTAB) was added toform self-assembly micelles for accelerating the recognition process. CH shows a 28-fold fluorescence enhancement ((I- I-0)/I-0 at 550 nm) over other biological species by optimizing the chemical recognition process of CH with alpha-KA. Significantly, CH was successfully applied for thefluorescence discrimination of all kinds of blood cancer serum samples. This work would provide a potential method that is quick and convenient for sensing alpha-KA and may promote fluorescence detection in clinical diagnosis. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.