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论文类型： 期刊论文

发表时间： 2017-08-15

发表刊物： BIOSENSORS & BIOELECTRONICS

收录刊物： SCIE、EI、PubMed、Scopus

卷号： 94

页面范围： 536-543

ISSN号： 0956-5663

关键字： Two-photon; NIR emission; Ultra-low detection limit; Hydrogen peroxide; Cell imaging

摘要： Hydrogen peroxide (H2O2), one of the reactive oxygen species (ROS), plays vital roles in diverse physiological processes. Imbalance of the H2O2 is concerned with serious diseases such as cardiovascular disorders, neurodegenerative diseases, Alzheimer's disease and cancer. Therefore, it is critical to develop efficient methods for monitoring H2O2 in vivo. In this work, a two-photon excitation (860 nm) NIR fluorescent turn-on probe TPNR-H2O2 for H2O2 based on Dicyanomethylene-4H-pyran fluorophore is reported, which can be used in solution detection with 13.2-fold NIR fluorescence enhancement, fast response (completed within 40 min), excellent sensitivity (DL 72.48 nM), and lower cellular auto-fluorescence interference. Importantly, the perfect photostability of TPNR-H2O2 clearly demonstrated that the probe could be applied to imaging intracellular H2O2 for a long time without photobleaching. In addition, through two-photon imaging, this probe was cell permeable and used to monitor the level of endogenous and exogenous H2O2 with promising biological application.