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论文类型： 期刊论文

发表时间： 2017-07-01

发表刊物： SENSORS AND ACTUATORS B-CHEMICAL

收录刊物： SCIE、EI、Scopus

卷号： 246

页面范围： 293-299

ISSN号： 0925-4005

关键字： Hypochlorous acid; Ratiometric probe; Completely separated fluorescent signals; Lysosomes

摘要： Hypochlorous acid (HClO), as one of the reactive oxygen species (ROS), was reported to be involved in a number of human diseases and many efforts have therefore been made to probe its biological roles at molecular level. In this work, a ratiometric probe (BFCIO) with completely separated fluorescent signals consisting of a boron-dipyrromethene (BODIPY) dye conjugated with Fisher aldehyde.was designed and synthesized for the selective and sensitive detection of HOCI via oxidative cleavage of the alkene linker. The probe evinced more than 300-fold enhancement in fluorescence ratio(I-511nm/I-713nm) in the presence of HClO over other ROS with fast response time (within 10 s) and low detection limit (10.6 nM). Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane and visualize exogenous and endogenous HOCI in lysosomes of living cells. (C) 2017 Elsevier B.V. All rights reserved.