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论文类型：期刊论文

发表时间：2017-01-01

发表刊物：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY

收录刊物：SCIE

卷号：19

期号：5

页面范围：1259-1264

ISSN号：1560-8530

关键字：Lily symptomless virus; TGB1; RNA silencing suppressor; Subcellular localization

摘要：Lily symptomless virus (LSV) is one of the major viral diseases affecting lilies. Many plants have an innate antiviral response that includes viral RNA silencing. To counter this pathway, plant viruses have developed RNA silencing suppressor proteins. However, it is not known whether LSV encodes an RNA silencing suppressor. This study was undertaken to characterize the function of LSV movement protein TGB1 and unknown protein 16kDa and to determine whether LSV encodes an RNA silencing suppressor protein. A viral GFP expression system was used to determine whether16 kDa was able to inhibit RNA silencing, inhibit local and systemic GFP silencing and reverse systemic RNA silencing. However, 16 kDa did not inhibit local GFP silencing triggered by dsRNA in an agrobacterium co-infection assay in transgenic Nicotiana benthamiana (16c) carrying GFP. TGB1 did not inhibit gene silencing and appeared to function only as a movement protein. Neither 16 kDa nor TGB1 acted as a virulence factor in N. benthamianais infected with PVX. Results further showed that 16 kDa and TGB1 localized to the nucleus and cytomembrane. This is the first report to show that LSV 16 kDa plays a critical role in RNA silencing suppression is not a virulence factor and has a clear subcellularlocalization pattern. Future studies are required to fully elucidate the function of 16 kDa in antiviral response in plants. (C) 2017 Friends Science Publishers