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Date of Publication:2013-01-01
Journal:环境科学与技术
Volume:36
Issue:3
Page Number:83-88
ISSN No.:1003-6504
Abstract:Biodegradation of dye wastewater is becoming a hot research field in
recent years. Through gradient acclimation, a novel strain C-2 was
isolated from soil in the laboratory, which could decolorize Acid Red
GR, the sulfonated azo dyes effectively. The strain C-2 was identified
as Acinetobacter sp. by 16S rDNA sequence analysis. The optimal
conditions of decolorization estimated by response surface methodology
(RSM) were as 17.5 g·L~(-1) of strain C-2, 100 mg·L~(-1) of initial GR
concentration, 2.50 g·L~(-1) of yeast extract and 3.00 g·L~(-1) of
fructose. Under the optimal conditions, the maximal decolorization
efficiency was 99% within 14h. The decolorization kinetics was fitted
well with Haldane equation, and the maximum specific decolorization
rate, affinity constant and inhibition constant were found to be 328.33
mg·(g·h)~(-1), 309.69 mg·L~(-1) and 1,365.83 mg·L~(-1) respectively. The
calculated optimal specific decolorization rate and GR concentration
were 105.91 mg·(g·h)~(-1) and 650.37 mg·L~(-1). The results suggested
that the strain C-2 with highly decolorizing activity could be useful
for the practical bioremediation.
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