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Date of Publication:2012-01-01

Journal:应用与环境生物学报

Volume:18

Issue:5

Page Number:873-879

ISSN No.:1006-687X

Abstract:As a type of bacterial aromatic ring-cleavage enzymes with versatile
   functions, extradiol dioxygenases (EDOs) have broad application in the
   field of environmental protection, chemical synthesis and
   biotechnologies. This paper reviewed the achievements of EDOs researches
   including the studies on their classification, catalytic mechanism, the
   application in biodegradation, biosynthesis, biotechnologies and the
   novel methods for extracting and modify EDOs. EDOs belong to three
   evolutionarily independent families, which utilize Fe(II) or Mn(II) as
   metal ions to catalyze the oxygenolytic fission of aromatic compounds
   via iron-alkylperoxo intermediates by the incorporation of substrates
   and O_2. Through this mechanism, EDOs possess broad substrates spectra,
   and often take part in the biosynthesis of active compounds. Meanwhile,
   EDOs play an important role in the biotechnology such as biosensors.
   Recently, combined with the technology of metagenomic, hybrid enzymes,
   more and more EDOs were extracted and modified to provide a general
   message for the in depth study of EDOs. Fig 5, Ref 32

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