Indexed by:期刊论文
Date of Publication:2021-02-01
Journal:CELL DEATH & DISEASE
Volume:10
Issue:5
Page Number:343
ISSN No.:2041-4889
Key Words:CCAAT enhancer binding protein beta; fructose 6 phosphate; glutamine fructose 6 phosphate aminotransferase; growth arrest and DNA damage inducible protein 153; hexosamine; n acetylglucosamine; nicotine; vimentin, acylation; Article; breast cancer; breast carcinogenesis; breast epithelium; cancer growth; cell differentiation; cell migration; cellular stress response; cigarette smoking; controlled study; endoplasmic reticulum stress; epithelial mesenchymal transition; gene silencing; human; human cell; priority journal; promoter region; protein expression; protein phosphorylation; repressor gene; smoking; transcription regulation
Abstract:Cigarette smoking has been shown to be a carcinogenic factor in breast cancer. Nicotine (Nic), an active component of tobacco, has been found to induce epithelial-mesenchymal transition (EMT) in breast cancer cells. However, the alterations in protein O-GlcNAcylation in Nic-mediated tumorigenesis and malignization mechanisms are less well studied. Herein, we found that cellular O-GlcNAcylation dramatically increased in human breast cancer cells with EMT activation induced by Nic. Elevated O-GlcNAcylation subsequently promoted Nic-induced EMT activation and increased cell migratory abbility. In addition, we demonstrated that a differentiation factor for the mammary epithelium, CCAAT/enhancer-binding protein B (CEBPB), was involved in Nic-induced hyper-O-GlcNAcylation via transcriptional regulation of the expression of the key enzyme glutamine: fructose-6-phosphate amidotransferase (GFAT) and thus increased the flux through the hexosamine biosynthetic pathway (HBP). Finally, elevated O-GlcNAcylation of the transcriptional repressor C/EBP homologous protein (CHOP) suppressed its heterodimerization with CEBPB and facilitated the DNA-binding activity of CEBPB, further generating positive feedback that enhanced EMT upon Nic stimulation. In conclusion, our results have revealed a new regulatory mechanism involving CEBPB/GFAT-induced hyper-O-GlcNAcylation that plays a key role in EMT and smoking-mediated breast cancer progression.
Associate Professor
Supervisor of Master's Candidates
Gender:Female
Alma Mater:吉林大学
Degree:Doctoral Degree
School/Department:化工海洋与生命学院
Discipline:Pharmacology
Business Address:F03-311
Contact Information:0427-2631427
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