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HPLC分析四种獐牙菜中芒果苷的质量分数

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Indexed by:期刊论文

Date of Publication:2009-11-15

Journal:大连工业大学学报

Included Journals:ISTIC

Volume:28

Issue:6

Page Number:401-403

ISSN No.:1674-1404

Key Words:HPLC;獐牙菜;芒果苷

Abstract:采用HPLC分析了四种獐牙菜中芒果苷的质量分数.色谱柱为SinoChrom ODS-BP C18(4.6 mm×250 mm,5 μm);流动相为乙腈-水[V(水):V(磷酸):V(NaOH)=1000:0.2:1];流动相乙腈体积分数变化为10%~25%(0~10 min),25%(10~35 min),30%(35~45 min),30%~65%(45~60 min);体现流量1 mL/min;检测波长254 nm.结果表明,芒果苷在 0.024 5~0.122 5 mg/mL线性关系良好.滇獐牙菜、川东獐牙菜、北方獐牙菜和抱茎獐牙菜的芒果苷质量分数分别为0.023 4%,0.014 4%,0.061 6%,0.044 8%.该分析方法简单,精密度,重现性好,且杂质干扰少,样品分析的稳定性和回收率较高,可有效用于测定獐牙菜中芒果苷的质量分数.

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