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Indexed by:期刊论文

Date of Publication:2017-01-01

Journal:NEOPLASMA

Included Journals:SCIE

Volume:64

Issue:5

Page Number:681-692

ISSN No.:0028-2685

Key Words:SZC014; oleanolic acid derivative; apoptosis; breast cancer cells

Abstract:Oleanolic acid (OA) and its derivatives are a novel emerging class of compounds. Although OA exhibits potent anticancer and anti-inflammatory function, the potential effect of its new derivatives (SZCO14) in human breast cancers has not been understood yet. In this investigation, we demonstrated the anticancer effect of SZCO14, a novel OA derivative and identified the possible mechanisms by which SZCO14 induced MCF-7 cell death. The biological functions of SZCO14 were validated by MTT, migration and colony formation assays in breast cancer cells. Cell apoptosis was monitored by Annexin V-FITC assay. Intracellular ROS and cell cycle were measured by flow cytometric analysis. Western blot was used to detect protein expression level. Our present results fully demonstrated that SZCO14 inhibits breast cancer cells proliferation, colony formation, and cell migration. Further investigation verified that ROS generation, apoptosis induction and Go/G, phase arrest was observed in SZCO14-treated MCF-7 cells. However, pretreatment with N-acetyl- L-cysteine (NAC), a ROS scavenger, increased the expression of procaspase-3. Additionally, SZCO14 treatment suppressed the levels of Akt, phosphorylated-Akt (p-Akt), COX-2, p-p65 in the cytoplasmic and p65 in nuclear. Furthermore, the inhibition of p65 nuclear translocation was confirmed by immunofluorescence staining. These data show that SZCO14 is an effectively selective anticancer agent against breast cancer cells, highlighting the potential use of this derivative as a breast cancer therapeutic agent.