Release Time:2019-10-10  Hits:

Indexed by: Journal Article

Date of Publication: 2007-03-01

Journal: PHYTOCHEMICAL ANALYSIS

Included Journals: PubMed、SCIE

Volume: 18

Issue: 2

Page Number: 91-97

ISSN: 0958-0344

Key Words: fingerprinting; Daphne genkwa; yuanhuacine; quantitative analysis; HPLC-UV

Abstract: Daphne genkwa contains a novel class of anticancer diterpene esters that inhibit DNA topoisomerase I. Fingerprint and quantitative analysis by HPLC were performed in order to characterise and evaluate D. genkwa. A standard fingerprint of Daphne diter-pene esters from the root extract was first established by HPLC-LJV, and the major peaks in the fingerprint profile were preliminarily determined using HPLC-MS. The principal Daphne diterpene esters, yuanhuacine (1), yuanhuadine (2), yuanhuajine (3) and yuanhuagine (4), were isolated and identified using a combination of UV, IR, MS, H-1-NMR and C-13-NMR spectral data. Quantitative analysis indicated that 1 was the principal component in the root, and that 2 was the major component in the buds. The average extraction rates of 1 and 2 were 0.0151 and 0.0033% (n = 10) from the root, respectively, and 0.0020 and 0.0078% (n = 3) from the buds. respectively. Copyright (c) 2006 John Wiley & Sons, Ltd.