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论文类型：期刊论文

发表时间：2013-10-15

发表刊物：BIOCHEMICAL ENGINEERING JOURNAL

收录刊物：SCIE、EI、Scopus

卷号：79

页面范围：144-152

ISSN号：1369-703X

关键字：Affinity; Microporous membranes; Chromatography; Bioseparations; Bilirubin; Bile salt

摘要：Bilirubin (BR) adsorbents have low removal efficiency because of the tight binding of BR with human albumin (HA) in a complicated blood system. Sodium deoxycholate (SDC) was selected as an adsorption promoter to improve the BR adsorption capacity of a cellulose acetate (CA)/polyethyleneimine (PEI) membrane. Static adsorption experiments show that the maximum BR adsorption capacity of the membrane with SDC in BR-HA mock solution is 100-200% at the molar ratio of SDC to HA ranging from 8 to 12, higher than that without SDC. It is also found that SDC is more efficiently adsorbed by the membrane than BR and HA. Absorption, circular dichroism, and zeta potential studies demonstrate that SDC can be bound with the BR-HA complex to form a ternary BR-HA-SDCm complex. On the basis, the facilitated adsorption mechanism of BR with SDC was proposed that SDC aggregates or micelles form a quasi-multilayer adsorption on the membrane, increase approachable binding sites, and prolong the distance between the BR-HA complex and the membrane. Thus, SDC as a spacer reduces the influence of the steric hindrance of HA, resulting in an enhanced BR adsorption capacity. Dynamic adsorption results further evidence the facilitated adsorption mechanism. (C) 2013 Elsevier B.V. All rights reserved.