赵建章
Personal Homepage
Publications
Current location: Home >> Research >> Publications
In situ formation of SERS hot spots by a bis-quaternized perylene dye: a simple strategy for highly sensitive detection of heparin over a wide concentration range.
Hits:

Indexed by:期刊论文

Date of Publication:2018-01-01

Journal:The Analyst

Included Journals:PubMed、SCIE

Volume:143

Issue:8

Page Number:1899-1905

ISSN No.:1364-5528

Abstract:A simple and ultrasensitive surface-enhanced Raman scattering (SERS) assay for heparin detection has been demonstrated based on an in situ hot spot assembly method using N,N'-bis[3,3'-(trimethylammonium)propylamine]-3,4,9,10-perylenediimide (bis-quaternized perylene dye, abbreviated as BQPER). In this assay, a BQPER probe with two positive charges is employed as both Raman reporter and electrostatic bridge to cause the aggregation of citrate-capped Ag nanoparticles (Ag NPs), resulting in a strong SERS signal due to the formation of numerous hot spots in the gaps of the aggregates. The SERS intensity of BQPER decreases in the presence of heparin, because BQPER binds primarily to heparin due to its high anionic charge to mass ratio compared to that of Ag NPs. Accordingly, a novel method for the detection of heparin has been developed based on SERS of the unbound BQPER probe. Two obvious advantages of the proposed heparin assay over other reported methods are: its much wider linear concentration range (10-5-10-10 g mL-1) and lower limit of detection (0.08 ng mL-1). Besides, our proposed heparin assay has been successfully applied to the detection of heparin in serum samples, indicating its great potential in the diagnosis of heparin-related diseases. This work provides a new Raman probe for the SERS analysis of heparin and opens a different perspective for SERS-based transducer design in charged analytes.

Personal information

Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates

Gender:Male

Alma Mater:Jilin University

Degree:Doctoral Degree

School/Department:School of Chemistry Engineering

Discipline:Organic Chemistry. Applied Chemistry. Physical Chemistry (including Chemical Physics)

Business Address:西部校区E座208房间

Contact Information:0411-84986236

Click:

Open time:..

The Last Update Time:..


Address: No.2 Linggong Road, Ganjingzi District, Dalian City, Liaoning Province, P.R.C., 116024

MOBILE Version