赵建章
Personal Homepage
Publications
Current location: Home >> Research >> Publications
Synthesis of novel bispyrene diamines and their application as ratiometric fluorescent probes for detection of DNA
Hits:

Indexed by:期刊论文

Date of Publication:2009-08-15

Journal:BIOSENSORS & BIOELECTRONICS

Included Journals:SCIE、EI、PubMed

Volume:24

Issue:12

Page Number:3442-3447

ISSN No.:0956-5663

Key Words:DNA; ctDNA; Fluorescence probe; Pyrene; Ratiometric

Abstract:Fluorescent DNA probes with 1,6-hexanediyl as the linker between two pyrenes, phenylpyrenes or phenylethynyl pyrene fluorophores were synthesized (Py-1, Py-2 and Py-3) and their interactions with DNA were studied by UV-vis absorption spectra, fluorescence spectra and viscosity measurements. The probes show red-shifted emission compared with pyrene (up to 20 nm). We found the interaction of these probes with DNA can be either intercalation or groove binding. Ratiometric fluorometry (ratio of the monomer and excimer emission intensity versus concentration of DNA) was achieved with these probes for DNA quantification (with limit of detection, LOD, up to 0.1 mu g/mL). We also found that the undesired oxygen sensitivity of the emission intensity of pyrene fluorophore can be greatly suppressed by extending the pi-conjugation framework of pyrene (the I(Ar)/I(air) value is decreased from 8.10 for pyrene to less than 2.20 for the DNA probes described herein). (C) 2009 Elsevier B.V. All rights reserved.

Personal information

Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates

Gender:Male

Alma Mater:Jilin University

Degree:Doctoral Degree

School/Department:School of Chemistry Engineering

Discipline:Organic Chemistry. Applied Chemistry. Physical Chemistry (including Chemical Physics)

Business Address:西部校区E座208房间

Contact Information:0411-84986236

Click:

Open time:..

The Last Update Time:..


Address: No.2 Linggong Road, Ganjingzi District, Dalian City, Liaoning Province, P.R.C., 116024

MOBILE Version