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发布时间：2019-03-13

论文类型：期刊论文

发表时间：2019-01-28

发表刊物：CHEMISTRY-A EUROPEAN JOURNAL

收录刊物：Scopus、PubMed、SCIE

卷号：25

期号：6

页面范围：1498-1506

ISSN号：0947-6539

关键字：cysteine; iridium; phosphorescence; sensing and imaging; time-gated luminescence

摘要：This study reports an activatable iridium(III) complex probe for phosphorescence/time-gated luminescence detection of cysteine (Cys) in vitro and in vivo. The probe, [Ir(ppy)(2)(NTY-bpy)](PF6) [ppy: 2-phenylpyridine; NTY-bpy: 4-methyl-4 '-(2-nitrovinyl)-2,2 '-bipyridine], is developed by incorporating a strong electron-withdrawing group, nitroolefin, into a bipyridine ligand of the Ir-III complex. The luminescence of the probe is quenched owing to the intramolecular charge transfer (ICT) process, but switched on by a specific recognition reaction between the probe and Cys. [Ir(ppy)(2)(NTY-bpy)](PF6) shows high sensitivity and selectivity for Cys detection and good biocompatibility. The long-lived emission of [Ir(ppy)(2)(NTY-bpy)](PF6) allows time-gated luminescence analysis of Cys in cells and human sera. These properties make it convenient for the phosphorescence and time-gated luminescence imaging and flow cytometry analysis of Cys in live samples. The Cys images in cancer cells and inflamed macrophage cells reveal that [Ir(ppy)(2)(NTY-bpy)](PF6) is distributed in mitochondria after cellular internalization. Visualizations and flow cytometry analysis of mitochondrial Cys levels and Cys-mediated redox activities of live cells are achieved. By using [Ir(ppy)(2)(NTY-bpy)](PF6) as a probe, in vivo sensing and imaging of Cys in D. magna, zebrafish, and mice are then demonstrated.