Title of Paper:Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

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Date of Publication:2011-08-01

Journal:CHINESE JOURNAL OF CHEMICAL ENGINEERING

Included Journals:ISTIC、SCIE、EI

Volume:19

Issue:4

Page Number:682-686

ISSN No.:1004-9541

Key Words:2,3-butanediol; Jerusalem artichoke; salting-out extraction; viscosity

Abstract:The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate. 2,3-Butanediol, an important chemical used widely is also a main product of sugar-based fermentation carried out by Klebsiella pneumoniae. In this study, we investigated the use of salting-out extraction (SOE) that employed a K(2)HPO(4)/ethanol system consisting of 21% ethanol and 17% K(2)HPO(4) (mass fraction) to separate 2,3-butanediol from the viscous Jerusalem artichoke-based fermentation broth. After SOE, about 98% of solid matters was removed, and the viscosity decreased from 72.5 mPa.s in the original fermentation broth to 4.4 mPa.s in the top phase. The partition coefficient and yield of 2,3-butanediol reached 13.4 and 99%, respectively, and 89% of soluble proteins was removed from the broth. The results showed that SOE is an efficient way for isolating 2,3-BD from a highly viscous fermentation broth by removing much of the solid matters within the broth.