Title of Paper:AI-2 Key Enzyme S-Ribosylhomocysteinase from Strain Klebsiella pneumoniae CICC 10011 Producing 2,3-Butanediol

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Date of Publication:2011-03-01

Journal:CHEMICAL RESEARCH IN CHINESE UNIVERSITIES

Included Journals:Scopus、SCIE、ISTIC

Volume:27

Issue:2

Page Number:273-276

ISSN No.:1005-9040

Key Words:S-Ribosylhomocysteinase(LuxS); Kinetics; Characterization; Klebsiella pneumoniae

Abstract:S-Ribosylhomocysteinase(LuxS) is the key enzyme in the synthetic pathway of a quorum sensing autoinducer AI-2. LuxS from a 2,3-butanediol produced strain Klebisella pneumoniae CICC 10011 was cloned and characterized. The luxS gene is composed of 540 bp with 172 amino acids encoded. The K(m) value for S-ribosylhomocysteine(SRH) was (27 +/- 1) mmol/L, k(cat) was (0.112 +/- 0.004) s(-1) and k(cat) /K(m) was 4.4x10(3) L(.)mol(-1.)s(-1) at 25 degrees C. LuxS was activated by divalent metal ions, the highest activity was detected with Co(2+) form, followed by Mg(2+), Ba(2+), Mn(2+), Fe(2+) and Ca(2+), and activation constant for Co(2+) is (16 +/- 2) mu mol/L.