董铭铭，副教授，博导。2007年于南开大学获学士学位，2010年于南京大学获硕士学位，2016年于中国科学院大学获博士学位，2018年-2020年于美国约翰霍普金斯大学医学院从事博士后研究。主要研究兴趣包括：基于液相色谱-质谱联用的翻译后修饰蛋白质组学新方法与新技术开发；将多组学新技术与临床生物学问题相结合，解析重大疾病的发生发展机制；将组学新技术转化应用于生物医学领域重要疾病的临床标志物的高通量筛选、表征及验证；利用组学技术支持新型生物医用材料的开发。累计发表SCI 论文60余篇，以第一作者/通讯作者在Nature Chemical Biology, Nature Communications (2), Advanced Science(2), Analytical Chemistry (7), Trends in Analytical Chemistry 等本领域重要国际期刊上发表论文24篇。主持国家自然科学基金面上项目、国家自然科学基金青年基金、大连市高层次人才创新创业项目-大连市青年科技之星、大连理工大学引进人才科研启动项目等项目，参与国家自然科学基金委重点项目、国家自然科学基金委中加健康研究合作计划等项目。入选辽宁省百千万人才工程万人层次。

欢迎具有生物工程、生物化工、化学、生物等背景的研究生报考。

发表第一作者及通讯作者（含共同）文章目录：

(1)  A Protein-Centric Strategy Coupled with Match-Between-Run Glycoproteomics Enables Discovery of Robust Site-Specific Glycan Biomarkers for Hepatocellular Carcinoma, Advanced Science, 2026, 0, e16299 （中科院一区，top）

(2) Library-based virtual match-between-runs quantification in GlyPep-Quant improves site-specific glycan identification, Nature  Communication, 2025, 16, 6483（中科院一区，top）

(3) A label-free method by combining chromatography enrichment with N-terminal specific proteolysis enables sensitive identification of Tn/sTn glycosites, Analytical Chemistry, 2025, 97, 15038-15048（中科院一区，top）

(4) Microparticle-assisted protein capture method facilitates proteomic and glycoproteomic analysis of urine samples, Anal. Chim. Acta, 2025, 1335, 343448

(5) Modulation of core-fucosylation oxidation for selective enrichment and characterization of cell surface glycoprotein, ACS Chemical Biology, 2025, 20, 2979−2990

(6) A novel method for Tn antigen detection based on chemoenzymatic targeted labelling and CuAAC click chemistry, Chemical Communications, 2026, 62, 2599

(7) Temporal resolved multi‑proteomic analysis enabled the systematic characterization of N‑glycosylation pattern changes during Jurkat T cell activation, Analytical Bioanalytical. Chemistry, 2025, 417, 2169-2183

(8) Quantitative Characterization of Protein N-Linked Core-Fucosylation by an Efficient Glycan Truncation Strategy, Analytical Chemistry, 2024, 96, 10506−10514 (1) （中科院一区，top）

(9)  Rational development of functional hydrophilic polymer to characterize site-specific glycan differences between bovine milk and colostrum, Food Chemistry, 2024, 460,140669（中科院一区，top）

(10) Robust Glycoproteomics Platform Reveals a Tetra-Antennary Site-Specific Glycan Capping with Sialyl-Lewis Antigen for Early Detection of Gastric Cancer, Advanced Science, 2024,11, 2306955（中科院一区，top）

(11) O-Glycopeptide Truncation Strategy for Heterogeneous O-GalNAc Glycoproteomics Characterization, Analytical Chemistry, 2023, 95, 10017−10024（中科院一区，top）

(12) Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O-Linked Intact Glycopeptides, Analytical Chemistry, 2023, 95, 7448−7457（中科院一区，top）

(13) Glyco-Decipher: glycan database-independent peptide matching enables discovery of modified glycans and in-depth characterization of site-specific N-glycosylation, Nature Communication, 2022, 13(1), 1900 （中科院一区，top）

(14) Data Independent Acquisition-based Mass Spectrometry (DIA-MS) for the quantitative analysis of N-linked glycopeptides, Analytical Chemistry, 2021, 93, 13774−13782（中科院一区，top）

(15) Urinary glycoproteins associated with aggressive prostate cancer, Theranostics, 2020, 10(26): 11892-11907（中科院一区，top）

(16) Development of Parallel Reaction Monitoring Assays for the Detection of Aggressive Prostate Cancer Using Urinary Glycoproteins, Journal of Proteome Research, 2021, 20, 3590−3599

(17)  The divide and conquer strategies for deep phosphoproteomics analysis, Trends in Analytical Chemistry, 2018, 105: 282-291 （中科院一区，top）

(18)  SH2 Superbinder Modified Monolithic Capillary Column for the Sensitive Analysis of Protein Tyrosine Phosphorylation, Journal of Proteome Research, 2018, 17, 243−251 

(19)  Sensitive, Robust, and Cost-Effective Approach for Tyrosine Phosphoproteome Analysis, Analytical Chemistry, 2017, 89(17):9307-9314. （中科院一区，top）

(20)  Ultra-deep tyrosine phosphoproteomics enabled by a phosphotyrosine superbinder, Nature Chemical Biology, 2016, 12, 959–966. （中科院一区，top）

(21) Selective Enrichment of Cysteine-Containing Phosphopeptides for Subphosphoproteome Analysis, Journal of Proteome Research, 2015, 5341-5347

(22)  Identification of phosphopeptides with unknown cleavage specificity by a de novo sequencing assisted database search strategy, Proteomics, 2014, 14, 2410-2416

(23) Depletion of Acidic Phosphopeptides by SAX To Improve the Coverage for the Detection of Basophilic Kinase Substrates, Journal of Proteome Research, 2012, 11, 4673-4681

(24)  Coupling Strong Anion-Exchange Monolithic Capillary with MALDI-TOF MS for Sensitive Detection of Phosphopeptides in Protein Digest, Analytical Chemistry, 2010, 82, 2907–2915（中科院一区，top）